Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (1): 74-77.doi: 10.3969/j.issn.1673-8225.2011. 01.016

Previous Articles     Next Articles

Combination of basic fibroblast growth factor and nerve growth factor affects the differentiation of adult rat hippocampal neural stem cells into neuron-like cells

Tong Lei, Ji Li-li, Xie Da-long, Gao Hai, Tong Xiao-jie   

  1. Department of Human Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang  110001, Liaoning Province, China
  • Received:2010-07-25 Revised:2010-09-10 Online:2011-01-01 Published:2011-01-01
  • Contact: Tong Xiao-jie, Doctor, Professor, Doctoral supervisor, Department of Human Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang 110001, Liaoning Province, China xjtong@mail.cmu.edu.cn
  • About author:Tong Lei☆, Doctor, Department of Human Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang 110001, Liaoning Province, China tonglei@mail.cmu.edu.cn
  • Supported by:

    the Specific Scientific Research Foundation of Doctor Subject Center of Higher Learning Schools, No. 20070159016; the Natural Science Foundation of Liaoning Province, No. 20082097

PDF

463

Abstract:

BACKGROUND: Differentiation of neural stem cells (NSCs) was mediated by many factors. Several factors could induce NSCs to differentiate into neurons in varying degrees and it is now a focus on the control of NSCs differentiation.  
OBJECTIVE: To study the effects of combination of basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) on the differentiation of adult rat hippocampal NSCs into neurons.
METHODS: The rat brain hippocampus was removed sterilely. Cell suspension was prepared and diluted when the diameter of the fourth passage of clone sphere was 200 μm by mixture of DMEM/F12 containing 2% B27, 20 µg/L epidermal growth factor (EGF) and 20 µg/L bFGF. Monoclonal cells were passaged. NSCs were divided into blank control, bFGF, NGF and bFGF+NGF groups. Immunocytochemistry for identification of NSCs was done to detect positive rate of neuron specific enolase (NSE) and the differentiation of NSCs into neurons.  
RESULTS AND CONCLUSION: ①The monoclonal cells expressed nestin and the differentiated cells expressed NSE and glial fibrillary acidic protein. ②The proportions of NSE-positive cells in bFGF group, NGF group and bFGF+NGF group were much higher than in blank control group (P < 0.05), with the highest in bFGF+NGF group (P < 0.05). These indicate that bFGF and NGF can elevate the differentiation of NSCs into neurons, and their combination obtains better outcomes.

CLC Number: